The Effect of Colcemid on the Heat Survival of Mitotic V79 Chinese Hamster Cells

Authors

J. G. Szekely, Whiteshell Laboratories
G. P. Raaphorst, Ottawa Regional Cancer Centre
A. U. Lobreau, Whiteshell Laboratories
S. Delaney, Whiteshell Laboratories
E. I. Azzam, Ottawa Regional Cancer Centre

Abstract

V79 Chinese hamster cells were collected by colcemid addition to study the effect of heat on mitosis. When they were heated at 42°C and 45°C in the presence of 0.06 μg/mL colcemid, cell survival increased over the control samples, which were heated in ordinary medium. Scanning electron microscopy showed that cells heated to 45°C in the presence or absence of colcemid had fewer microvilli on the surface, but they did not have increased bleb formation. Transmission electron microscopy showed that the chromatin was diffuse in the heated cells and the kinetochores were indistinct. The mitochondria in the heated cells were also swollen and contained visible particles.

Recommended Citation

Szekely, J. G.; Raaphorst, G. P.; Lobreau, A. U.; Delaney, S.; and Azzam, E. I. (1991) "The Effect of Colcemid on the Heat Survival of Mitotic V79 Chinese Hamster Cells," Scanning Microscopy: Vol. 6: No. 1, Article 14.
Available at: https://digitalcommons.usu.edu/microscopy/vol6/iss1/14