Improved Visualization of DNA in Aqueous Buffer with the Atomic Force Microscope

Authors

Magdalena Bezanilla, University of California, Santa Barbara
Carlos J. Bustamante, University of Oregon, Eugene
Helen G. Hansma, University of California, Santa Barbara

Abstract

An improved method has been developed for imaging deoxyribonucleic acid (DNA) in aqueous buffer with the atomic force microscope (AFM). DNA on untreated mica can be imaged in aqueous buffer with the AFM if the DNA is deposited onto the mica in a buffer with HEPES and MgCl₂, if the sample is rinsed thoroughly with high water pressure, and if the imaging is done with an electron beam-deposited (EBD) tip that has been deposited in the scanning electron microscope (SEM). The water rinse removes DNA that is otherwise easily scraped off the substrate. There is evidence that sharper tips may be more damaging to DNA when imaged in aqueous buffer especially when the DNA is bound tightly to the mica. The ability to image DNA in nearly biological conditions has potential applications for imaging biomolecular processes with the AFM.

Recommended Citation

Bezanilla, Magdalena; Bustamante, Carlos J.; and Hansma, Helen G. (1993) "Improved Visualization of DNA in Aqueous Buffer with the Atomic Force Microscope," Scanning Microscopy: Vol. 7: No. 4, Article 2.
Available at: https://digitalcommons.usu.edu/microscopy/vol7/iss4/2