Low Temperature Embedding of Chemically Unfixed Biological Material After Cryosorption Freeze-Drying

Authors

L. Edelmann, Universität des Saarlandes

Abstract

After freeze-drying of bulk specimens in a newly developed cryosorption freeze-dryer (CFD) a special accessory is used to infiltrate the specimens in Lowicryl HM20 and to polymerize them at low temperature by ultra-violet (UV) irradiation within the CFD chamber in flat embedding moulds. The accessory allows polymerization in a dry, oxygen free environment without the risk of evaporation of volatile components of the resin which may lead to unsatisfactory polymerization. First results demonstrate the quality of structure preservation of biological material not treated with any chemical fixative.

Recommended Citation

Edelmann, L. (1994) "Low Temperature Embedding of Chemically Unfixed Biological Material After Cryosorption Freeze-Drying," Scanning Microscopy: Vol. 8: No. 3, Article 14.
Available at: https://digitalcommons.usu.edu/microscopy/vol8/iss3/14