Scanning Electron Microscopy and Energy Dispersive Spectroscopy Analysis of Calciotraumatic Lines in Rat Labial Dentin After Acute Exposure to Strontium Chloride

Authors

Hiroyuki Mishima, Nihon University School of Dentistry at Matsudo
Toshiro Sakae, Nihon University School of Dentistry at Matsudo
Yukishige Kozawa, Nihon University School of Dentistry at Matsudo

Abstract

Rats were given strontium chloride (SrCli) intraperitoneally at a dose of 500 mg/kg. The upper incisors were removed after injection of strontium. These incisors were studied by scanning electron microscopy - energy dispersive spectroscopy analysis (SEM-EDS) and light microscopy to examine the calciotraumatic lines of strontium in the rat incisor labial dentin.

At 24 hours after injection of strontium, the calciotraumatic response was observed in the predentin using hematoxylin and eosin (H-E) staining. At 5 days, three layers of calciotraurnatic lines were present in the labial dentin using an H-E staining and backscattered electron imaging in the SEM. The external layer consisted of unmineralized dentin, the intermediate layer of relatively unmineralized dentin, and the internal layer of unmineralized dentin. By SEM-EDS analysis, strontium was detected in these layers. The elemental dot map showed that the external and internal unmineralized layers had a low calcium content. The magnesium concentration was higher in the internal unmineralized layer than the external unmineralized layer.

Recommended Citation

Mishima, Hiroyuki; Sakae, Toshiro; and Kozawa, Yukishige (1995) "Scanning Electron Microscopy and Energy Dispersive Spectroscopy Analysis of Calciotraumatic Lines in Rat Labial Dentin After Acute Exposure to Strontium Chloride," Scanning Microscopy: Vol. 9: No. 3, Article 16.
Available at: https://digitalcommons.usu.edu/microscopy/vol9/iss3/16