Abstract
A rapid flow injection - electrospray ionization – mass spectrometry (FI-ESI-MS) method for the quantitative and qualitative analysis of norditerpenoid alkaloids in larkspur plants was developed. The FI-ESI-MS method was calibrated for alkaloid concentrations with larkspur plant samples against an existing Fourier transform infrared spectroscopy FTIR method. There was a strong correlation (r = 0.9926, r = 0.9891) between the FTIR and FI-ESI-MS methods. The relative standard deviation (RSD) for all measurements were ≤ 6.2 % except for the sample with the lowest concentration which was 19%. The sample throughput of the FI-ESI-MS method is much higher than the FTIR method due to simpler sample preparation, autosampling capabilities, and short analysis times. Finally, the FIA-ESI-MS can be used to qualitatively analyze the alkaloids in larkspur samples since the data allows the observation of all ions that comprise the alkaloid signals.
Recommended Citation
Gardner, Dale R.; Lee, Stephen T.; and Cook, Daniel
(2021)
"Rapid Quantitative Analysis of Toxic Norditerpenoid Alkaloids in Larkspur (Delphinium spp.) by Flow Injection - Electrospray Ionization – Mass Spectrometry,"
Poisonous Plant Research (PPR): Vol. 4, p. 10-19.
DOI: https://doi.org/10.26077/jy40-h384
Available at:
https://digitalcommons.usu.edu/poisonousplantresearch/vol4/iss1/2
Included in
Agricultural Science Commons, Analytical Chemistry Commons, Other Animal Sciences Commons