Description

The purpose of this study was to determine whether elevation in HawaiÔi affects the coquiÕs microhabitat use such as substrate choice and height off the forest floor and physiological metrics such as osmolality, oxidative status, and energy metabolites (glucose, free glycerol, and triglycerides).

Document Type

Dataset

DCMI Type

Dataset

File Format

.csv

Publication Date

12-16-2022

Funder

USDA, Animal and Plant Health Inspection Service (APHIS)

Utah State University Department of Biology

Utah State University Ecology Center

Publisher

Utah State University

Methodology

Collections occurred over one to three nights due to differences in collection difficulty among sites until approximately 20uL of total plasma volume (for subsequent physiology assays) was collected from ca. 30 individual frogs at each site. Frogs were collected from four low (m), seven medium (350-550 m), and five high elevation (>750 m) field sites. 606 individuals (75 females, 531 males) were collected in total. Once captured, frog body temperature was measured using a 1-mm diameter thermocouple sensor inserted into the cloaca, and the temperature of the substrate on which the frog was found was measured using an infrared thermometer gun. Substrate type on which the frogs were found fell into eight groups: branch, forest floor, leaf, leaf litter, log, rock, tree trunk, and other (e.g., man-made objects). The frogÕs height off the forest floor was estimated in half-meter increments, and ambient temperature was recorded using a portable weather device. From 25 May until 27 July 2021, three temperature data loggers (iButtons) were placed at 0 m, 1 m, and 2 m above the ground at each field site to record ambient temperature every 15 minutes. Coqui frogs were kept in separate 3.7 L plastic bags until collections were complete, at which point frogs were processed in the sequential order in which they were caught. We measured glucose using an Accu-Chek Active blood glucose meter. Oxidative status (dROMs) was measured using an assay kit (MC435, Diacron International, Italy), ?which detects levels of hydroperoxides that oxidize an alkyl-substituted aromatic amine (A-NH2) to assess chronic oxidative stress. Lipid-related energy metabolites (free glycerol and triglycerides) were measured using ?sequential enzymatic colour endpoint assays (F6428, T2449 and G7793, Sigma- Aldrich, Missouri, USA). Plasma osmolality was assessed using a vapor pressure osmometer (model 5100C; Wescor Inc., Logan, Utah, USA) to determine the hydration state of each frog.

Language

eng

Code Lists

See README

Disciplines

Biology

License

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.

Identifier

https://doi.org/10.26078/2yq1-5r98

Checksum

e2d1e4158b631663acd474967fcd8f67

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Additional Files

Coqui_MicrohabitatPhysiology_data.csv (99 kB)
0f4a62ae4f77db33467eca3bd501045a

ibutton_df.csv (8328 kB)
1393880ef1fd72f42276b91330f53003

README_CoquiMicrohabitatPhysiology.txt (5 kB)
697bb147b6edbcb61c4a8cf99e1037d5

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Biology Commons

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