Expression of the Agrobacterium Rhizogenes Rolc Gene in a Deciduous Forest Tree Alters Growth and Development and Leads to Stem Fasciation
We have altered the growth and development of a deciduous forest tree by transforming hybrid aspen (Populus tremula x Popu- lus tremuloides) with the Agrobacterium rhizogenes rolC gene ex- pressed under the strong cauliflower mosaic virus 35s promoter. W e demonstrate that the genetically manipulated perennial plants, after a period of dormancy, maintain the induced phenotypical changes during the second growing period. Furthermore, mass- spectrometrical quantifications of the free and conjugated forms of indole-3-acetic acid and cytokinins and severa1 gibberellins on one transgenic line correlate the induced developmental alterations such as stem fasciation to changes in plant hormone metabolism. We also show that the presence of the RolC protein increases the levels of the free cytokinins, but not by a process involving hydro- lysis of the inactive cytokinin conjugates.
Nilsson, Ove; Moritz, Thomas; Sundberg, Bjorn; Sandberg, Goran; and Olsson, Olof, "Expression of the Agrobacterium Rhizogenes Rolc Gene in a Deciduous Forest Tree Alters Growth and Development and Leads to Stem Fasciation" (1996). Aspen Bibliography. Paper 1681.