"Inheritance and linkage of allozymes and restriction fragment length p" by Z. Liu and G.R. Furnier
 

Aspen Bibliography

Inheritance and linkage of allozymes and restriction fragment length polymorphisms in trembling aspen

Document Type

Article

Journal/Book Title/Conference

Journal of Heredity

Volume

84

Issue

5

First Page

419

Last Page

424

Publication Date

1993

Abstract

Allozyme variants in leaf tissue of the parents and progeny of five full-sIb families of trembling aspen (Populus tremuloides; n = 19) were examined for nine enzyme systems. We consistently resolved allozymes of 14 loci, among which eight were polymorphic (Aco-1, Aco-2, Got-3, Per-1, Pgm-1, 6Pg-2, Pgi-2, and Skdh) and six were monomorphic (Got-1, Got-2, Mdh-1, Mdh-2, Pgl-1, and Sod). No significant deviation from the expected segregation ratios was observed for these allozyme loci. The results of joint two-locus segregation analyses indicated no linkage between 12 testable allozyme locus pairs. In one of these full-sib families of 93 progeny, we examined inheritance and linkage of four segregating allozyme loci and 75 RFLP loci corresponding to clones from an aspen Pst I genomic DNA library. None of the segregation ratios of the RFLP loci deviated significantly from those expected. We have constructed a linkage map that incorporates 54 RFLP and three allozyme loci comprising 14 linkage groups and covering 664 cM of the genome.

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