"A transcriptional roadmap to wood formation" by M. Hertzberg, H. Aspeborg et al.
 

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A transcriptional roadmap to wood formation

Document Type

Article

Journal/Book Title/Conference

Proceedings of the national academy of sciences, usa [proc. Natl. Acad. Sci. Usa]

Volume

98

Issue

25

First Page

14732

Last Page

14737

Publication Date

2001

Abstract

The large vascular meristem of poplar trees with its highly organized secondary xylem enables the boundaries between different developmental zones to be easily distinguished. This property of wood-forming tissues allowed us to determine a unique tissue-specific transcript profile for a well defined developmental gradient. RNA was prepared from different developmental stages of xylogenesis for DNA microarray analysis by using a hybrid aspen unigene set consisting of 2,995 expressed sequence tags. The analysis revealed that the genes encoding lignin and cellulose biosynthetic enzymes, as well as a number of transcription factors and other potential regulators of xylogenesis, are under strict developmental stage-specific transcriptional regulation.

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