Aspen Bibliography

Effect of different media and growth regulators on the in vitro shoot proliferation of aspen, hybrid aspen and white poplar male tree and molecular analysis of variants in micropropagated plants

Salah Khattab

Document Type

Article

Journal/Book Title/Conference

Life Science Journal

Volume

8

Issue

1

First Page

177

Last Page

184

Publication Date

2011

Abstract

Among other in vitro factors like temperature and light, concentration of plant growth regulators and medium constituents are two of the most important aspects of successful micropropagation. With the aim of optimization of in vitro multiplication of Populus alba L., Populus tremula L. and Populus tremula L. x Populus. tremuloides Michx , the effect of MS and WPM media with various concentrations of BAP and 2iP was studied. The following multiplication parameters were monitored: number of shoots regenerated/explant, explant height, and explants weight were determined. MS medium proved to be the most effective one, resulting in better and morphologically superior microshoots as compared to WPM medium in the case of Populus alba. However in Populus tremula and Populus tremula x Populus Tremuloides the highest number of shoots was found when grown on WPM medium. In all three poplar lines, the highest shoot multiplication was obtained on MS and WPM media supplemented with BAP at (0.1 and 0.2 mgl-1). Very poor multiplication was achieved on media with 2iP. Shoot tips were isolated and induced to root on MS medium supplemented with IAA, IBA and/or NAA (0.0, 0.1, 0.2 and 0.4 mg l -1). About 90% of the rooted plantlets tested have successfully established in soil. In vitro derived plants were genetically analyzed using RAPD fingerprints. RAPD analysis confirmed that all the in vitro derived plant which tested were genetically identical to their donor plants, suggesting the absence of detectable genetic variation in the regenerated plants.

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