Date of Award:

5-2012

Document Type:

Thesis

Degree Name:

Master of Science (MS)

Department:

Animal, Dairy, and Veterinary Sciences

Committee Chair(s)

Kamal Rashid

Committee

Kamal Rashid

Committee

Abby D. Benninghoff

Committee

Kerry A. Rood

Committee

Charles D. Miller

Committee

Kenneth L. White

Abstract

The Center for Integrated Biosystems (CIB) at Utah State University proposed to demonstrate the added benefits of bench top stirred-tank bioreactors utilizing the packedbed impeller system in perfusion mode for the production of recombinant proteins. In this study recombinant Chinese Hamster Ovary (CHO) cells were utilized for the production of a secreted protein in two bioreactor types: pitched-blade bioreactor operated in batch mode versus packed-bed bioreactor operated in perfusion mode. Our primary objective was to determine whether the packed-bed bioreactor is superior to a traditional pitched-blade bioreactor for the mass production of cell culture protein products. We hypothesize that the reduced culture stress and optimal growth conditions of the packed-bed basket system will result in a higher yield of protein product compared to the traditional pitched-blade system.

The pitched-blade impeller is commonly used for the growth of shear sensitive animal cells. The impeller provides gentle mixing of the media without damaging the cells. The packed-bed basket impeller is composed of two metal perforated screens. Inbetween the screens are Fibra-Cel disks which create a bed to which suspension cells are entrapped. Cells cultured in a packed-bed bioreactor are growing in a shear free environment as they are not exposed to hydrodynamic forces allowing for maximum growth and protein expression.

Three independent experiments were performed in each bioreactor system described. The results from these experiments could help investigators involved in protein and/or vaccine production facilities select the appropriate bioreactor and mode of operation to optimize cell productivity for a specific protein product.

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Comments

This work made publicly available electronically on July 31, 2012.

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