Date of Award:

5-1971

Document Type:

Thesis

Degree Name:

Master of Science (MS)

Department:

Nutrition, Dietetics, and Food Sciences

Department name when degree awarded

Nutrition and Food Sciences

Committee Chair(s)

D. K. Salunkhe (Committee Co-Chair), Bharat Singh (Committee Co-Chair)

Committee

D. K. Salunkhe

Committee

Bharat Singh

Committee

C. A. Ernstrom

Committee

G. H. Richardson

Abstract

The incorporation of 3-14C-,B-hydroxy-, B-methylglutaric acid (HMG) vii into carotenoids of excised etiolated maize shoots, crude cell free extracts and soluble extracts (20, 000 x g) of maize was investigated. HMG was effectively incorporated into carotenoids of excised shoots, crude extracts and soluble extracts. The excised shoots, crude extracts and soluble extracts incorporated 2-14C-mevalonic acid (MVA) into carotenoids as well. The results indicated the presence of HMG-CoA reductase in the plant as well as an HMG activating enzyme. The soluble extract showed a pH optimum of 7. 0 for incorporation of HMG into carotenoids. Endogenous metabolites such as MVA in the soluble enzyme preparation decreased the amount of 3-14C-HMG incorporated into carotenoids. The conversion of HMG to MVA may be a regulatory site in carotenoid biosynthesis in the plant.

Telone (a mixture of cis-1, 3-dichloropropene, trans-I, 3-dichloropropene and other halogenated hydrocarbons), 3-chloroallyl alcohol, 3-chloropropionic acid, 3-hydroxypropionic acid, 3-chloro-1-propanol and malonic acid significantly reduced the amount of 3-14C-HMG incorporated into carotenoids of maize.

Activity of lipoxidase from tomato extracts was significantly inhibited by Telone, cis-1, 3-dichloropropene, trans-1, 3-dichloropropene, 3-chloroallyl alcohol, 3-chloropropionic acid, 3-hydroxy-propionic acid, 3-chloro-1-propanol and malonic acid.

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