Date of Award:
5-1975
Document Type:
Thesis
Degree Name:
Master of Science (MS)
Department:
Nutrition, Dietetics, and Food Sciences
Department name when degree awarded
Nutrition and Food Sciences
Committee Chair(s)
Gary H. Richardson
Committee
Gary H. Richardson
Committee
Dr. Ernstrom
Committee
Dr. Mendenhall
Abstract
An automated continuous flow analysis for milk protein utilizing ultraviolet spectroscopy resulted in a correlation coefficient of 0.972 and a standard estimate of error of 0.082 percent protein when 30 samples were compared with the acid orange 12 dye binding method. Milk was solubilized in 95-105 volumes of acetic acid using the Technicon Auto Analyzer II and measured for absorbance at 274 nanometers. No deviation from the standard method due to mastitis and varying milk fat concentration was observed. A theoretical model indicated a possible standard estimate of error 0.059 percent protein due to inter- and intra-breed variation in milk protein fraction distribution. Potassium dichromate and other preservatives interferred with the results.
Checksum
300253336dc91576a5b937e1ac264f9b
Recommended Citation
Wilkinson, R. Ford, "Milk Protein Analysis by Automated Ultraviolet Spectroscopy" (1975). All Graduate Theses and Dissertations, Spring 1920 to Summer 2023. 5149.
https://digitalcommons.usu.edu/etd/5149
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