Practical Methods for Identification of Rice Endosperm Protein Bodies and Fecal Protein Particles in Light Microscopy

Authors

D. L. Barber
J. N. A. Lott
D. A. Harris

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 License.

Abstract

Indigestible 1-3 ~m protein 'cores', particles that originate from protein bodies of rice endosperm, were examined in bright-field and fluorescence microscopy, using a variety of histological and histochemical procedures. Our application of histological, histochemical and chemical approaches to a study of the feces of aoimals fed rice-containing diets has resulted in the development of methods for routine identification of insoluble rice fecal protein particles. For the specific identification of rice endosperm storage protein, whether protein bodies, cores or fecal protein particles, aqueous eosin Y stain in conjunction with fluorescence microscopy, and a combination of orange G + aniline blue in bright-field microscopy appear to be reliable methods when used in conjunction with each other.

Recommended Citation

Barber, D. L.; Lott, J. N. A.; and Harris, D. A. (1991) "Practical Methods for Identification of Rice Endosperm Protein Bodies and Fecal Protein Particles in Light Microscopy," Food Structure: Vol. 10: No. 2, Article 4.
Available at: https://digitalcommons.usu.edu/foodmicrostructure/vol10/iss2/4