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Description
Fibrin is a natural polymer already used in extreme clinical settings as an adhesive and for shallow wounds when activated by the enzyme thrombin. However, current formations contain irritants, are slow drying, and are not easily washed off. This study used a spray-on bandage composed of thrombin and fibrinogen on a monolayer of 3T3 mouse fibroblast cells to test the enhancement of the body's natural healing without the risks of current formulations. The viability and growth of scratched cells were analyzed using live/dead stain assays 0, 1, and 3 days post-treatment. The results of this study showed cell growth, fibrinogen formation, and scratching were successful. The bottles' spray exerted too much force and caused additional cell death compared to untreated cells. Additionally, the cells migrated too quickly through the scratch, resulting in rapid gap closure. Further research on cell scaffolding, bandage application, and cell viability is required for a successful spray-on bio-bandage.
Publication Date
12-3-2022
City
Logan, UT
Keywords
bio-bandage, cell growth, cell scaffolding, fibrin
Disciplines
Engineering
Recommended Citation
Peterson, Kristine; Moravek, Amanda; and Edward, Abby, "The Spray-On Bio-Bandage" (2022). Fall Student Research Symposium 2022. 2.
https://digitalcommons.usu.edu/fsrs2022/2