Date of Award
5-2013
Degree Type
Report
Degree Name
Master of Science (MS)
Department
Biological Engineering
Committee Chair(s)
H. Scott Hinton
Committee
H. Scott Hinton
Committee
Ronald C. Sims
Committee
Charles D. Miller
Abstract
The objective of this study is to optimize the growth media and genes to maximize PHB production computer simulations, and to evaluate the simulation results through experiments. In order to achieve this objective, the metabolism of E. coli that produces PHB was modeled using the existing E. coli genome-scale metabolic networks. To identify the best additional nutrient for PHB production, carbon sources, minerals, amiano acids, nitrogen sources, and sulfur sources were examined by production potential analysis. Strain design was conducted to find the unwanted reactions to overexpress PHB synthesis pathway.
Recommended Citation
Heshiki, Yoshitaro, "Optimization of Polyhydroxybutyrate Production in Recombinant Escherichia Coli Through Metabolic Modeling and Simulation" (2013). All Graduate Plan B and other Reports, Spring 1920 to Spring 2023. 291.
https://digitalcommons.usu.edu/gradreports/291
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