Date of Award
2013
Degree Type
Thesis
Degree Name
Departmental Honors
Department
Animal, Dairy, and Veterinary Sciences
Abstract
Using commercially available antibodies, we created a modified ELISA strip assay to determine pregnancy in cattle. The anti‐progesterone antibody was printed on small membrane strips via an Inkjet printer. The strips were subsequently analyzed using varying concentrations of progesterone. The objective of this study was to assess this novel antibody printing process and to determine the binding activity of the anti‐progesterone antibody. The long‐term objective is to develop a pen-side pregnancy test that would cost less than $1.00. In this specific study, it was determined that the anti‐progesterone antibody used did not provide adequate sensitivity for visualization required for pen‐side analysis. Additional studies will assess the sensitivity of other anti‐progesterone antibodies and the possible implementation of amplification steps.
Recommended Citation
Kelley, Alexandra Windley, "Analysis of an Inkjet Printed Strip Assay for Pregnancy Test in Cattle" (2013). Undergraduate Honors Capstone Projects. 139.
https://digitalcommons.usu.edu/honors/139
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Faculty Mentor
Lee Rickords