Class
Article
College
College of Engineering
Department
Biological Engineering Department
Faculty Mentor
Elizabeth Vargis
Presentation Type
Poster Presentation
Abstract
Scaffolds are used for several functions in tissue engineering, including providing structural support and promoting cell adhesion and differentiation. In this study, we examined the value of using onion epithelial membranes (OEM) as a scaffold for skin cell growth. We tested the growth of NIH 3T3 fibroblasts on decellularized and non-decellularized OEM and compared the cell growth to that in typical tissue flask culture conditions. We examined cell growth, viability, and attachment in each culture condition. We found that NIH 3T3 fibroblasts grew on the non-decellularized OEM, but the decellularized OEM was too fragile to use practically. Cell growth on the non-decellularized OEM was unable to be assessed and compared to tissue culture well plate controls as planned due to contamination. OEM have the potential to serve as a scaffold for connective tissue, but further work and adjustments to the methods are needed.
Location
Logan, UT
Start Date
4-14-2021 12:00 AM
Included in
Onion Epithelial Membranes as a Scaffold for Generating Connective Tissue
Logan, UT
Scaffolds are used for several functions in tissue engineering, including providing structural support and promoting cell adhesion and differentiation. In this study, we examined the value of using onion epithelial membranes (OEM) as a scaffold for skin cell growth. We tested the growth of NIH 3T3 fibroblasts on decellularized and non-decellularized OEM and compared the cell growth to that in typical tissue flask culture conditions. We examined cell growth, viability, and attachment in each culture condition. We found that NIH 3T3 fibroblasts grew on the non-decellularized OEM, but the decellularized OEM was too fragile to use practically. Cell growth on the non-decellularized OEM was unable to be assessed and compared to tissue culture well plate controls as planned due to contamination. OEM have the potential to serve as a scaffold for connective tissue, but further work and adjustments to the methods are needed.