The purpose of this study was to determine whether elevation in HawaiÔi affects the coquiÕs microhabitat use such as substrate choice and height off the forest floor and physiological metrics such as osmolality, oxidative status, and energy metabolites (glucose, free glycerol, and triglycerides).
USDA, Animal and Plant Health Inspection Service (APHIS)
Utah State University Department of Biology
Utah State University Ecology Center
Utah State University
Collections occurred over one to three nights due to differences in collection difficulty among sites until approximately 20uL of total plasma volume (for subsequent physiology assays) was collected from ca. 30 individual frogs at each site. Frogs were collected from four low (m), seven medium (350-550 m), and five high elevation (>750 m) field sites. 606 individuals (75 females, 531 males) were collected in total. Once captured, frog body temperature was measured using a 1-mm diameter thermocouple sensor inserted into the cloaca, and the temperature of the substrate on which the frog was found was measured using an infrared thermometer gun. Substrate type on which the frogs were found fell into eight groups: branch, forest floor, leaf, leaf litter, log, rock, tree trunk, and other (e.g., man-made objects). The frogÕs height off the forest floor was estimated in half-meter increments, and ambient temperature was recorded using a portable weather device. From 25 May until 27 July 2021, three temperature data loggers (iButtons) were placed at 0 m, 1 m, and 2 m above the ground at each field site to record ambient temperature every 15 minutes. Coqui frogs were kept in separate 3.7 L plastic bags until collections were complete, at which point frogs were processed in the sequential order in which they were caught. We measured glucose using an Accu-Chek Active blood glucose meter. Oxidative status (dROMs) was measured using an assay kit (MC435, Diacron International, Italy), ?which detects levels of hydroperoxides that oxidize an alkyl-substituted aromatic amine (A-NH2) to assess chronic oxidative stress. Lipid-related energy metabolites (free glycerol and triglycerides) were measured using ?sequential enzymatic colour endpoint assays (F6428, T2449 and G7793, Sigma- Aldrich, Missouri, USA). Plasma osmolality was assessed using a vapor pressure osmometer (model 5100C; Wescor Inc., Logan, Utah, USA) to determine the hydration state of each frog.
This work is licensed under a Creative Commons Attribution 4.0 License.
Beard, K. H., French, S. S., & Marchetti, J. (2022). Elevation Influences the Microhabitat Use and Baseline Physiology of Coqui Frogs in Hawai‘i [Data set]. Utah State University. https://doi.org/10.26078/2YQ1-5R98
Additional FilesCoqui_MicrohabitatPhysiology_data.csv (99 kB)
ibutton_df.csv (8328 kB)
README_CoquiMicrohabitatPhysiology.txt (5 kB)