Description
This dataset supports the production, characterization, and biological validation of a novel recombinant hagfish intermediate filament (rHIF) protein functionalized with spider silk motifs (rHIF-alpha-GRGGL). The data demonstrates a scalable bioprocess resulting in a 7.76 g/L yield, high structural alpha-helicity (77.2%), and significant support for N2a cells.
Author ORCID Identifier
Justin A. Jones https://orcid.org/0000-0002-3647-5361
Kristin Durrant https://orcid.org/0000-0002-4468-979X
Document Type
Dataset
DCMI Type
Dataset
File Format
.xlsx, .csv, .png, .docx, .pdf, .TIF, .bmp
Viewing Instructions
Special software required to use data: OriginPro was used for FTIR Gaussian deconvolution and generating fit reports.
Publication Date
3-24-2026
Publisher
Utah State University
Methodology
Data collection included monitoring upstream fermentation parameters including OD600 and Glucose levels. Downstream processing involved mass balance calculations for protein yield and densitometry for purification results. Structural analysis was conducted using FTIR spectroscopy with Gaussian deconvolution and peak assignments to determine secondary structure distribution. Biological validation involved N2a cell assays to measure attachment area, neurite outgrowth in microns, normalized ROS levels, and viability over 3rd and 7th days in vitro.
Start Date
12-12-2023
End Date
2-29-2024
Language
eng
Code Lists
see README
Disciplines
Biology | Ecology and Evolutionary Biology | Molecular Biology
License
This work is licensed under a Creative Commons Attribution 4.0 License.
Recommended Citation
Jones, J., & Durrant, K. (2026). Recombinant Hagfish Intermediate Filament Proteins Enable Neural Culture Substrates that Promote Neurite Extension and Reduce Oxidative Stress [Data set]. Utah State University. https://doi.org/10.26078/0R27-S605
Checksum
14561a45fd4fe4caa9342872f1191388