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Growth characteristics and biochemical changes of poplar shoots in vitro under sodium chloride stress

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Journal of Plant Physiology





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The effect of NaCl on poplar shoots grown in vitro was studied. After 28 days of cultivation on media containing up to 300 mmol/L NaCl, the shoot and root lengths as well as the rooting percentage were smaller than in the control. These effects were enhanced by increasing salinity. Changes in several biochemical markers (soluble proteins, soluble sugars and peroxidase activity) were observed in poplar shoots cultivated for 7 days on salt-supplemented and control media. Measurements showed that the soluble protein content of the plants decreased dramatically on day 1 but stabilized to a constant value thereafter, except for the 300 mmol/L NaCl condition. Peroxidase activity increased with salt content and time of cultivation, except for the 300 mmol/L NaCl condition, where it remained unchanged. Changes were also observed in the contents of glucose, fructose, sucrose and galactose. Glucose and fructose contents strongly increased after 3 days of cultivation on 300 mmol/L NaCl-containing medium. A maximum sucrose content was observed on day 1 for all conditions, followed by a decrease until day 7. Galactose was unaffected by the salt treatments.