Journal of Economic Entomology
A semidefined diet for the honey bee, Apis mellifera L., developed by Nation and Robinson (1966), resulted in egg-laying by the queen but supported larval development only to an age of 3-4 days. For larvae to develop beyond the age of 4 days and to pupate normally, at least 7.5% pollen had to be added. Nevertheless, when using 1 mg impure gibberellic acid (0.85 mg gibberellic acid + 0.15 mg impurities)/g of dry food was used, 390-520 cm2 of brood of all stages resulted in caged 3-frame-nucleus colonies. Also, bees fed gibberellic acid produced almost as much brood as the 1 colony fed 7.5% pollen. No replicates were used by Nation and Robinson (1966) in their test of 4 diets.
Barker, Roy J. and Taber, Stephen, "Evaluation of Gibberellic Acid in a Pollen Substitute for Honey Bees" (1973). An. Paper 214.
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