Title

Efficacy of soil applications of Metarhizium anisopliae (Metsch.) Sorokin conidia, and standard and lyophilized mycelial particles against scarab grubs

Document Type

Article

Journal/Book Title/Conference

Journal of Invertebrate Pathology

Volume

59

Issue

1

Publication Date

1-1-1992

First Page

54

Last Page

60

DOI

10.1016/0022-2011(92)90111-G

Abstract

The efficacy of soil inoculations of Metarhizium anisopliae (Metsch.) Sorokin propagules against third-instar grubs of the Japanese beetle and European chafer was investigated in the laboratory. Inoculum consisted of conidia; standard (unlyophilized mycelial particles with size ranges of <125, 125–250, and 250–500 μm; and lyophilized mycelial particles with size ranges of 125–250 μm and 250–500 μm. Grub mortality occurred significantly quicker in mycelium-inoculated compared with conidia-inoculated soil. Total mortality, however, did not differ significantly between the two types of inoculum. Pathogen titers were consistently higher in soil inoculated with 250- to 500-μm-size compared to 125- to 250-μm-size particles. Lyophilization of mycelial particles did not significantly affect soil titers or pathogenicity in two of the three bioassays conducted.

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