Electrophoretic detection of multiple protein kinases in the entomopathogenic fungus Metarhizium anisopliae
Archives of Microbiology
Multiple membrane bound protein kinases were detected following electrophoresis of a membrane preparation from Metarhizium anisopliae on sodium dodecyl sulfate-polyacrylamide gels. After renaturation, polypeptides in the gel were incubated with [γ-32P]ATP for phosphorylation of the kinases themselves or of histones or casein included in the gel. Eight radioactive bands were detected in ungerminated conidia, a 40 kDa band predominated. Sixteen bands (Mr range 28–72 kDa) were detected in both a crude extract and membrane fraction from mycelia. All of the bands were intensified when casein was included in the gel; four bands including the major 40-kDa band were intensified with mixed histones. The same bands were detected when the phosphorylation was carried out with or without Ca2+, calmodulin, EGTA, cyclic AMP, spermine or heparin. Most of the phosphorylating enzymes fit the general descriptions of casein kinases.
St. Leger, R.J., D.W. Roberts and R.C. Staples. 1990. Electrophoretic detection of multiple protein kinases in the entomopathogenic fungus Metarhizium anisopliae. Archiv. Microbiol. 154: 518-520.