Studies on the prolonged storage of Metarhizium anisopliae conidia. Effect of growth substrate on conidial survival and virulence against mosquitoes
Journal of Invertebrate Pathology
Metarhizium anisopliae was grown on six complex mycological media and on three types of rice at three moisture levels to determine the effect of growth substrate on conidial yield, viability, and virulence against mosquitoes immediately after spore maturation and after the storage of conidia at four different temperature-relative humidity (RH) combinations over a 1-year period. Conidial yields varied with the mycological media, but the viability and virulence of conidia against mosquitoes produced on all substrates were similar when spores were stored under the same conditions. The storage conditions were more critical to spore survival and virulence than the substrate upon which conidia were produced. The comparison of rice types for conidial production indicated that conidial yield, viability, and virulence to mosquitoes were more dependent upon the moisture level during growth and on the storage conditions that upon the rice used. The best storage conditions among those tested for the retention of both spore viability and virulence against mosquitoes were 19°C–97% RH and 4°C–0% RH.
Daoust, R.A. and D.W. Roberts. 1983. Studies on the prolonged storage of Metarhizium anisopliae conidia. Effect of growth substrate on conidial survival and virulence against mosquitoes. J. Invertebr. Pathol. 41:161 70.