Title

RNA polymerase activity of Amsacta moorei entomopox virions

Document Type

Article

Journal/Book Title/Conference

Intervirology

Volume

5

Publication Date

1-1-1975

First Page

69

Last Page

75

DOI

10.1159/000149882

Abstract

Optimum conditions for in vitro RNA synthesis by the entomopoxvirus from Amsacta moorei, except for a temperature optimum of 26 degrees, were similar to those reported for vaccinia. Incorporation of 3H-ATP in the presence of CTP, GTP and UTP was significantly inhibited by actinomycin D; incorporation of 3H-ATP alone was not. The products formed by incorporation of 3H-ATP alone or with the three other nucleotides contained polyadenylic acid sequences. The sedimentation coefficient of the 3H-ATP-labeled product formed in the presence of CTP, GTP and UTP was reduced from 8-23S to 3-5S after RNase treatment. The product formed from 3H-ATP alone had an apparent sedimentation value of 3-5S.

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