Title

Characterization of entomopox virons of the army cutworm, Euxoa auxiliaris (Lepidoptera: Noctuidae)

Document Type

Article

Journal/Book Title/Conference

Journal of Invertebrate Pathology

Volume

25

Issue

2

Publication Date

3-1-1975

First Page

215

Last Page

220

DOI

10.1016/0022-2011(75)90071-3

Abstract

Virions were released from virus-containing inclusions (VCI) of an entomopoxvirus of the army cutworm, Euxoa auxiliaris, with carbonate-thioglycolate solution. Knoblike projections present on the surface of the viral envelope were removed by digestion with trypsin. Trypsin-treated virions were homogeneous in both sucrose and CsCl gradients. The virions were similar to vertebrate poxviruses in morphology, contained 1.13 ± 0.3% DNA and had a buoyant density of 1.261 ± 0.003 gm/cm3 in CsCl. The virion preparations were infective and possessed RNA polymerase activity. Of eight species of Lepidoptera tested, only the species from which the virus was originally isolated proved susceptible to infection.

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