Dehiscence of Coelomomyces Psorophorae Sporangia From Aedes Taeniorhynchus: Induction by Amines and Amino Acids
Journal of Invertebrate Pathology
The effect of various chemicals on induction of dehiscence of nonsterile Coelomomyces psorophorae sporangia from Aedes taeniorhynchus larvae was examined. Tests were made with various salts, reducing agents, chelating agents, buffers, alcohols, carbohydrates, fatty acids and derivatives, amino acids and derivatives, peptides, amines, purines and pyrimidines, antibiotics, and plant hormones. The most active compound was Tris [tris(hydroxymethyl)aminomethane]. Tris was most effective at pH 8–9, and a concentration of 1–20 mM. Structure-activity studies indicated that active compounds had a basic requirement for NH2 and either COOH or CH2OH attached to the alpha carbon, but only certain amino acids and amines were highly active. Preparations virtually free of host debris were not responsive to Tris, but addition of bacteria-free homogenates of A. taeniorhynchus larvae restored responsiveness. Dehiscence of C. psorophorae sporangia from Psorophora howardii was also enhanced by exposure to Tris.
Roberts, D.W., M. Shapiro and R.L. Rodin. 1973. Dehiscence of Coelomomyces psorophorae sporangia from Aedes taeniorhynchus: Induction by amines and amino acids. J. Invertebrate Pathology 22: 175 181.