Toxins From the Entomogenous Fungus Metarrhizium Anisopliae: Isolation of Destruxins From Submerged Cultures
Journal of Invertebrate Pathology
Destruxins A and B, two depsipeptides toxic to insects, were present in submerged cultures of a North American isolate of Metarrhizium anisopliae. The fungus produced additional, unidentified compounds which also induced tetanic paralysis in larvae of the greater wax moth, Galleria mellonella. An isolation technique using carbon tetrachloride extraction of unfiltered liquid cultures as the initial step (in place of charcoal adsorption from culture filtrates) was developed to facilitate isolation of destruxins from large volumes of medium.
Roberts, D.W. 1969. Toxins from the entomogenous fungus Metarrhizium anisopliae: Isolation of destruxins from submerged cultures. J. Invertebrate Pathology 14: 82-88.