Efficient Screening of High-Signal and Low-Background Antibody Pairs in the Bio-Bar Code Assay Using Prion Protein as the target
Noroviruses are recognized worldwide as the principal cause of acute, non-bacterial gastroenteritis, resulting in 19-21 million cases of disease every year in the United States. Noroviruses have a very low infectious dose, a short incubation period, high resistance to traditional disinfection techniques and multiple modes of transmission, making early, point-of-care detection essential for controlling the spread of the disease. The traditional diagnostic tools, electron microscopy, RT-PCR and ELISA require sophisticated and expensive instrumentation, and are considered too laborious and slow to be useful during severe outbreaks. In this paper we describe the development of a new, rapid and sensitive lateral-flow assay using labeled phage particles for the detection of the prototypical norovirus GI.1 (Norwalk), with a limit of detection of 107 virus-like particles per mL, one hundred-fold lower than a conventional gold nanoparticle lateral-flow assay using the same antibody pair.
Brooks BD, Alberston AE, Jones JA, Speare JO, Lewis RV. Efficient screening of high-signal and low-background antibody pairs in the bio-bar code assay using prionprotein as the target, AnalyticalBiochemistryNov 1;382(1):60-2 Epub 2008 Jul 17.