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PCR-based fetal sex determination by use of fetal DNA in maternal plasma in bovine is a novel scientific method that has some cost advantages in the animal production industry. In this study, our objective was to determine fetal sex in pregnant cows and establish the lower boundary at which Y-chromosome specific fetal DNA could be detected in the circulating maternal plasma using Bov97M primers. In this experiment, our results showed that 10 pg was the limit in which the Y-chromosome specific DNA was detectable in the circulating maternal plasma. Future experiments will be conducted to determine the earliest gestational age at which fetal sex can be determined.

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