In the Spring of 2016, two deer showing clinical signs of neurological illness were reported to the Utah Division of Wildlife Resources. The animals were euthanized and post-mortem tissues collected for identification of any disease causing agents. Brain and spleen samples were sent to Dr. Eric Delwart, University of California-San Francisco for metagenomics evaluation. Mobukvirus (described once) and novel picornavirus and bovine parvovirus were tentatively identified. However, cell culture of the viruses is necessary for further characterization and genomic sequencing. If these novel viruses can be cultured, it may be possible to develop serologic tests for additional field investigations in wildlife. We (USU) received tissue samples in June and have started viral evaluation in a number of cell lines. The cell lines being evaluated include: MDBK cells (bovine kidney), MDCK cells (canine kidney), BHK-21 cells (hamster kidney), Vero cells (African green monkey kidney), ST cells (swine testis), SK-RST cells (swine kidney), RD Cells (human rhabdosarcoma), Hela cells (human adenocarcinoma), and the mosquito C636 cell line. In this experiment MDBK, SK-RST, and ST cells were used. Lipofection was performed to produce more virus using viral RNA extracted from the brain tissue. Supernatant from the lipofection was used to infect fresh confluent cells. Supernatant from the first infection were used to infect more cells using dilutions. The results are inconclusive as CPE has not been clearly characterized within the samples.
Winkler, Brennan; Moscon, Justin; Dewart, Eric; Roug, Annette; and Tarbet, Bart, "Culturing Viruses from Wild Deer in Utah" (2017). Biology Posters. Paper 168.