The Uintah Basin periodically experiences high ozone levels during the winter season (Lyman et al). Carbonyl compounds are produced from various emission sources and are precursors to ground level ozone production. Of these compounds, several aldehydes and ketones are volatile and listed as hazardous under the Clean Air Act (1990). We regularly measure carbonyl concentrations in air via high-performance liquid chromatography (HPLC) as part of our efforts to better understand and improve air quality in the Uintah Basin. For this project, we investigated possible improvements to our HPLC system to increase resolution, identification, and quantification of carbonyls in collected air samples. The goals of this work were to (1) achieve better separation of individual carbonyls to do so we investigated the impact of changes to flow rate/pressure and eluent composition to the method. (2) improve the signal/noise ratio of our measurements. To address the second objective we determined detection limits for the modified instrument configuration by modifying sample injection volumes
O'Neil, Trevor and Lyman, Seth, "HPLC Method development and instrument QC for Aldehyde and Ketone compounds" (2017). Biology Posters. Paper 191.