Bordetella avium has been isolated from turkeys showing clinical signs of respiratory disease and increased mortality in Sanpete County Utah, despite receiving the B. avium vaccine. To determine if recent B. avium isolates are related, or unrelated to the vaccine strain, twenty-five isolates from different time periods and different locations in the U.S. were collected for comparison by MALDI-TOF mass spectroscopy. Spectra were evaluated by MALDI Biotyper software (Bruker Co.) to determine relationships among the clinical isolates. Cluster analysis of the spectra showed four major clusters using the principle component scores for the three spectral peaks in highest abundance. These clusters also accounted for >70% of the variability in the data based on identification score values. Four of five Utah isolates were in the same cluster as the vaccine strain. However, one isolate from Utah and isolates from other locations did not cluster with the vaccine strain.
Tarbet, Bart; Barney, J.; McMillan, L.; Mason, A.; Gonder, E.; Temple, L.; and Frame, D., "Analyses of Bordetella isolates collected from turkeys with respiratory disease using MALDI-TOF mass spectroscopy and comparison to a Bordetella avium vaccine" (2016). Biology Posters. Paper 38.