Mechanistic Studies on Enzyme-Catalyzed Phosphoryl Transfer
Advances in Physical Organic Chemistry
Phosphoryl transfer reactions have essential roles throughout biochemistry. The enzymes that catalyze these reactions result in tremendous rate enhancements for their normally unreactive substrates. This fact has led to great interest in the enzymatic mechanisms, and debate as to whether the mechanisms for enzyme-catalyzed hydrolysis of phosphate esters differ from those of uncatalyzed reactions. This review summarizes the uncatalyzed reactions of monoesters, diesters and triesters. A selection of enzymatic phosphoryl transfer reactions that have been the most studied and are the best understood are discussed, with examples of phosphatases, diesterases, and triesterases.
A. C. Hengge . “Mechanistic Studies on Enzyme-Catalyzed Phosphoryl Transfer.” Advances in Physical Organic Chemistry, 2005, 40: 49-108.