Comment on "Structural Evidence for a Dynamic Metallocofactor During N2 Reduction by Mo-Nitrogenase"
American Association for the Advancement of Science (AAAS)
Kang et al. (Reports, 19 June 2020, p. 1381) report a structure of the nitrogenase MoFe protein that is interpreted to indicate binding of N2 or an N2-derived species to the active-site FeMo cofactor. Independent refinement of the structure and consideration of biochemical evidence do not support this claim.
Peters, J. W., Einsle, O., Dean, D. R., DeBeer, S., Hoffman, B. M., Holland, P. L., and Seefeldt, L. C. (2021) Comment on “Structural evidence for a dynamic metallocofactor during N2 reduction by Mo-nitrogenase.” Science 371, eabe5481.