Low Temperature Embedding

Authors

E. Carlemalm, University of Basel
W. Villiger, University of Basel
J. -D. Acetarin, University of Basel
E. Kellenberger, University of Basel

Abstract

The Lowicryl resins K4M and HM20 are methacrylate/acrylate based formulations which can re used for embedding biological material at low temperature in conjunction with either the progressive lowering of temperature (PLT) technique or with freeze-substitution. The resins are applicable over a very extended temperature range, approximately 210°K to 340°K. Even lower temperatures down to ca. 190°K can be reached with two new resins, K11M and HM23. Test embeddings of unfixed material after freeze-substitution have given promising results which could re useful for imnunocytochemical labeling. Lipid extraction is small or absent when the two new resins are used in combination with freeze-substitution.

Recommended Citation

Carlemalm, E.; Villiger, W.; Acetarin, J. -D.; and Kellenberger, E. (1985) "Low Temperature Embedding," Scanning Electron Microscopy: Vol. 4: No. 1, Article 16.
Available at: https://digitalcommons.usu.edu/electron/vol4/iss1/16