Scanning Electron Microscopy
Volume 4, Number 1 (1985) The Science of Biological Specimen Preparation for Microscopy and Microanalysis
Articles
Physics of the Preparation and Observation of Specimens that Involve Cryoprocedures
E. Kellenberger, E. Carlemalm, and W. Villiger
The State of Water in the Cell
William Negendank
Two Opposing Theories of the Cell: Experimental Testing by Cryomethods and Electron Microscopy
L. Edelmann
Cell Volume Regulation Studies with the Electron Microprobe
Mary Jo Ingram and F. Duane Ingram
Glutaraldehyde Fixation Chemistry A Scheme for Rapid Crosslinking and Evidence for Rapid Oxygen Consumption
Timothy J. A. Johnson
Demonstrable Fixative Interactions
Keith M. Meek and John A. Chapman
In Vivo Light Microscopy of Organs
Robert S. McCuskey
On the Application and Possibilities of In Vivo Microscopy in Liver Research
E. Wisse and R. S. McCuskey
Tandem Scanning Reflected Light Microscopy
Mojmir Petran, Milan Hadravsky, Alan Boyde, and Martin Mueller
Complementary Replicas of Ultra-Rapidly Frozen Specimens
M. J. Costello, R. D. Fetter, and T. G. Frey
Cryofixation and Cryosubstitution for Routine Work in Transmission Electron Microscopy
H. Sitte, K. Neumann, and L. Edelmann
The Glass Knife in Ultramicrotomy
Lahja Sevéus and Cecilia Tarras-Wahlberg
Cryoultramicrotomy and Electron Microanalysis of Isolated Bovine Adrenal Chromaffin Cells
Richard L. Ornberg
Problems Associated with the Preparation of Cell Suspensions for X-Ray Microanalysis Highlighted by the Comparison of Results with Those Obtained from Tissue Sections
A. Warley, M. D. Kendall, and I. W. Morris
Low Temperature Embedding
E. Carlemalm, W. Villiger, J. -D. Acetarin, and E. Kellenberger
A New Approach to Low Temperature Embedding: Quick Freezing, Freeze-Drying and Direct Infiltration in Lowicryl K4M
R. Chiovetti, S. A. Little, J. Brass-Dale, and L. J. McGuffee
Cryopreparation of Tissue for Electron Microscopy
John G. Linner, Stephen C. Bennett, Donna S. Harrison, and Alton L. Steiner
Freeze Substitution and Low Temperature Embedding
B. Humbel and M. Müller
Observation of Colloidal Gold Labelled Platelet Surface Receptors and the Underlying Cytoskeleton Using High Voltage Electron Microscopy and Scanning Electron Microscopy
R. M. Albrecht, J. A. Oliver, and J. C. Loftus
Detection of Small (5-15nm) Gold-Labelled Surface Antigens Using Backscattered Electrons
Paul Walther and Martin Müller
Colloidal Gold Particles Detected on Highly Structured Surfaces of Large Samples by Backscattered Electrons in the Scanning Electron Microscope
D. Studer and R. Hermann
Does the Embedding Chemistry Interact with Tissue?
B. E. Causton
To Resin or Not to Resin in Immunocytochemistry
J. J. Wolosewick
Plasma Membrane Antigens Detected by Replica Techniques
H. Hohenberg, W. Bohn, G. Rutter, and K. Mannweiler
The Morphology of Thin Metal Coatings Formed by Rotary Shadowing Biological Species in High Vacuum
J. A. Panitz
Immuno-Cytochemistry with Backscattered Electrons
D. Soligo, E. de Harven, M. T. Nava, and G. Lambertenghi-Deliliers
Human Metaphase Chromosome Preparation for Scanning Electron Microscopy - A Consideration of Inherent Problems
T. D. Allen, E. M. Jack, C. J. Harrison, D. Claugher, and R. Harris
This is the proceedings of the 4th Pfefferkorn Conference. Held March 25 to 30, 1985 at the Grand Canyon Squire Inn, Grand Canyon, AZ.