Immunocytochemical Labeling of Enzymes in Low Temperature Embedded Plant Tissue: The Precursor of Glyoxysomal Malate Dehydrogenase is Located in the Cytosol of Watermelon Cotyledon Cells

Authors

C. Sautter, TU München, Weihenstephan

Abstract

The Lowicryl-technique in combination with protein A gold was used in order to localize the precursor of glyoxysomal malate dehydrogenase in watermelon cotyledons. Preservation of the antigen was evaluated by a preembedding technique in isolated organelles. The glyoxysomal malate dehydrogenase was localized in tissue sections by a postembedding technique. Antigens of glyoxysomal malate dehydrogenase were found in the glyoxysomal matrix and in the cytosol, whereas the endoplasmic reticulum was completely free of labeling. Controls are presented by preimmunserum, by a serum against various proteins of the glyoxysomal membrane and by application of cycloheximide in order to inhibit translation at cytosolic ribosomes. The results are compared with immunocytochemical localizations of other plant microbody enzymes and of plant storage proteins.

Recommended Citation

Sautter, C. (1985) "Immunocytochemical Labeling of Enzymes in Low Temperature Embedded Plant Tissue: The Precursor of Glyoxysomal Malate Dehydrogenase is Located in the Cytosol of Watermelon Cotyledon Cells," Scanning Electron Microscopy: Vol. 4: No. 1, Article 24.
Available at: https://digitalcommons.usu.edu/electron/vol4/iss1/24