Date of Award:
Master of Science (MS)
Animal, Dairy, and Veterinary Sciences
Robert W. Sidwell
Tests in vitro for antiviral activity against avian influenza viruses, A/Turkey/Sanpete/85 (H6N8) and A/Turkey/Sanpete/86 (H10N9), isolated in Sanpete County, Utah, utilized known antiviral agents, amantadine•HCl (adamantanamine hydrochloride) and ribavirin (1-β-D ribofuranosyl-1,2,4-triazole-3-carboxamide). The testing involved evaluation of seven drug concentrations. Maximum tolerated dose, minimum inhibitory concentration and therapeutic indexes were determined for each drug used. Both drugs demonstrated reasonable antiviral activity. Then they were tested against the H10N9 strain in 10-day-old Broad White turkey poults. Large- and small- particle aerosol delivery methods utilized. Various parameters, including water and feed consumption, health score (based on visible signs), recoverable lung virus, lung hemagglutination titer, and visual lung scoring were considered for the quantification of viral infection and, thus, antiviral activity in birds. Recovery of viable virus from infected turkey poults proved sporadic. Visible signs were varied and mild, limiting the usefulness of health scores. The onset of illness was reflected in the decline in water consumption. Moderate antiviral activity was demonstrated with the use of ribavirin small-particle aerosol, but ribavirin fog was not effective. Amantadine product slight antiviral activity when used with both delivery systems. The potential efficacy of both drugs and application methods indicate further study of these control systems is warranted.
Call, Evan W., "Evaluation of Antiviral Compounds Against Avian Influenza" (1991). All Graduate Theses and Dissertations. 4072.
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