Date of Award:
5-1984
Document Type:
Thesis
Degree Name:
Master of Science (MS)
Department:
Animal, Dairy, and Veterinary Sciences
Committee Chair(s)
Thomas D. Bunch
Committee
Thomas D. Bunch
Committee
J. L. Shupe
Committee
N. C. Leone
Committee
E. J. Gardner
Committee
W. C. Foote
Abstract
A reliable technique for obtaining late prophase, prometaphase and greater numbers of metaphase chromosomes is presented. The increased number of mitoses and high resolution chromosomes were obtained using a modification of the typical three-day blood culture technique in which the cell cycle was temporarily blocked at the G1-s border with methotrexate. A high resolution G-band idiogram is presented using a system similar to the standardization of human chromosomes. The use of this technique and system of nomenclature will facilitate routine analyses of horse chromosomes and provide a basis for the identification and recording of chromosome aberrations in this species. C-bands and NORs of the domestic horse are also described.
C-bands, NORs and high resolution G-bands of control horses were compared with those of horses affected with Hereditary Multiple Exostosis. No differences were found on NORs, C- and G-bands between control and exostosis horses.
Checksum
6c8c98ab27b650af742051fd4619c192
Recommended Citation
Maciulis, Alma, "Detailed Description and Nomenclature of High Resolution G-Banded Horse Chromosomes and Cytogenetic Analysis of Hereditary Multiple Exostosis in Horses" (1984). All Graduate Theses and Dissertations, Spring 1920 to Summer 2023. 4157.
https://digitalcommons.usu.edu/etd/4157
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