Date of Award:
Doctor of Philosophy (PhD)
Biological and Irrigation Engineering
The development of a general approach for non-destructive chemical and biological functionalization of materials could expand opportunities for both fundamental studies and creating various device platforms. Phage display has emerged as a powerful method for selecting peptides that possess enhanced selectivity and binding affinity toward a variety of targets. In this study, a powerful yet benign approach for identifying binding motifs to materials like (Poly) dimethylsiloxane, epoxy, and (Poly) ethylenetetraphthalate and peptide nanotubes has been demonstrated via comprehensively screened phage-displayed peptides. Further, along with the development of microstructures, micropatterns and micro-molecular self-assembly, recognition with phage-displayed peptides can be specifically localized in these microstructures.
In addition, the development of a facile approach for fabricating a library of precisely positioned nanostructures and microfluidic systems based on mammalian hair offers exciting opportunities in fundamental research and practical applications. The current top-down and bottom-up nanofabrication methods have been restricted in accessibility in standard labs due to their high cost and complexity. Novel fabrication methods utilizing biomimetic materials and natural proteins for large-scale nanopatterning with hierarchical assembly of functional materials have been reported. It is anticipated that these results could open up exciting opportunities in the use of peptide-recognized materials in fundamental biochemical recognition studies, as well as in applications ranging from analytical storage devices, hybrid materials, sensors, surface and interface, to cell biology.
Swaminathan, Swathi, "Bio-Inspired Materials and Micro/Nanostructures Enabled by Peptides and Proteins" (2015). All Graduate Theses and Dissertations. 4223.