Date of Award:
Doctor of Philosophy (PhD)
Randolph V. Lewis
Two major hurdles face the production of recombinant spider silk protein (rSSp) based materials. First, the production of sufficient quantities of rSSp has proven difficult due to their highly repetitive nature and protein size (>250kDa). Secondly, rSSp and native silks are practically insoluble in water based solutions, necessitating the use of harsh organic solvents that can remain in the material after production. While others are working on solving production problems, this dissertation demonstrates a novel aqueous solubilization method that is rapid (<1 minute) and results in near 100% solubilization of the rSSp. From this new solubilization method films, foams, gels (hydrogels and lyogels), adhesives, coatings and fibers have been produced as well as the previously unreported sponge. All of these novel materials were derived from entirely aqueous solutions with and without minor additives to influence the final physical state of the rSSp.
Jones, Justin A., "Aqueous Solvation Method for Recombinant Spider Silk Proteins" (2015). All Graduate Theses and Dissertations. 4267.
Copyright for this work is retained by the student. If you have any questions regarding the inclusion of this work in the Digital Commons, please email us at .