Date of Award:

1969

Document Type:

Thesis

Degree Name:

Doctor of Philosophy (PhD)

Department:

Nutrition, Dietetics, and Food Sciences

Advisor/Chair:

E. B. Wilcox

Abstract

Male castrated rats treated with testosterone or estradiol were given a diet containing 9, 18, or 50 per cent protein. Total lipids, lipid classes and fatty acid composition of lipid classes were determined.

Estradiol treated rats grew slower, smaller and consumed less diet. Their liver weights were larger when compared to their body weights. Moisture content of liver remained unaffected by sex hormones, but increased with the high level of protein in the diet.

Both testosterone and estradiol affected lipid metabolism; in general, the estrogenic influence was more pronounced and more predictable. Estradiol administration increased total lipids, free and esterified cholesterol and phospholipids in serum. Liver triglyceride level was decreased and free fatty acids and phospholipids were increased.

An effect of sex hormone on the percentage composition of serum and liver fatty acids was apparent. Estradiol dosed rats maintained higher proportions of myristic and strearic acids in the serum cholesterol esters than that of the other fatty acids. It also increased the level of stearic and oleic acids and decreased the level of myristic, palmitic and linoleic acids in serum phospholipids. Oleic acid concentration was increased while linoleic acid concentration was decreased in serum triglycerides of estradiol treated rats. The liver cholesterol ester fatty acid patterns remained unchanged by sex hormone treatement. Liver phospholipid fractions contained a lower percentage of oleic acid in estradiol treated groups. Testosterone and estradiol treated rats had a higher proportion of myristic, stearic and linoleic acids and lower proportion of oleic acid in liver triglycerides than did the control rats. Dietary content of protein did not change serum lipid values significantly. The high level of dietary protein decreased oleic acid concentration in liver phospholipids and triglycerides.

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