Date of Award:
5-2019
Document Type:
Dissertation
Degree Name:
Doctor of Philosophy (PhD)
Department:
Biological Engineering
Committee Chair(s)
Elizabeth Vargis
Committee
Elizabeth Vargis
Committee
Scott Hinton
Committee
Jon Takemoto
Committee
Charles Miller
Committee
Yu Huang
Abstract
Age-related macular degeneration (AMD) is a major cause of blindness in adults. Abnormal growth of blood vessels in the eye during the course of AMD causes damage to the retina, resulting in irreversible blindness. The goal of this research was to determine whether physical pressure on retinal cells can contribute to the increased blood vessel formation. To replicate the tears in the cell layers, a micropatterning method was used as a means of detaching cells from each other. Two new devices were also developed to mimic slow and fast increases in mechanical pressure on cell layers of the eye. After detaching cells from each other and adding mechanical stress to cells, the levels of angiogenic proteins secreted by retinal cells were measured. The results showed that both cell-cell detachment and mechanical stress can increase the secretion of angiogenic proteins. After adding mechanical stress, we also added the secreted proteins to blood vessel cells and observed an increase in blood vessel formation, indicating that mechanical stress can independently induce angiogenesis. These results suggest that physical stimuli in the eye can contribute to the aberrant blood vessel formation in AMD.
Checksum
5ca58c32099c8aeff3ca9dae6c579873
Recommended Citation
Farjood, Farhad, "Effect of Physical Stimuli on Angiogenic Factor Expression in Retinal Pigment Epithelial Cells" (2019). All Graduate Theses and Dissertations, Spring 1920 to Summer 2023. 7457.
https://digitalcommons.usu.edu/etd/7457
Copyright for this work is retained by the student. If you have any questions regarding the inclusion of this work in the Digital Commons, please email us at .