The Observation of the Displacement of Emulsifiers by Confocal Scanning Laser Microscopy

Authors

I. Heertje, Unilever Research Laboratorium
J. Nederlof, Unilever Research Laboratorium
H.A.C.M. Hendrickx, Unilever Research Laboratorium
E. H. Lucassen-Reynders, Unilever Research Laboratorium

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Abstract

The stability of food emulsions is an important aspect of the processing and the shelf life of many food systems. In order to control the stability, knowledge of the competitive adsorption of different emulsifiers is required. Current methods to study the displacement of emulsifiers from interfaces are rather laborious. A new method has been developed which is based on the observation of a planar interface between oil and water by fluorescent Confocal Scanning Laser Microscopy (CSLM). The displacement of fluorescein isothio-cyanate- labeled sodium caseinate by monoacylglycerols and proteins has been studied. Quantitative information on the competitive adsorption as a function of the type of emulsifier and the concentration has been obtained. The methodology offers a new approach to studying the effects of emulsifiers and dynamic processes in the stabilization of emulsions.

Recommended Citation

Heertje, I.; Nederlof, J.; Hendrickx, H.A.C.M.; and Lucassen-Reynders, E. H. (1990) "The Observation of the Displacement of Emulsifiers by Confocal Scanning Laser Microscopy," Food Structure: Vol. 9: No. 4, Article 4.
Available at: https://digitalcommons.usu.edu/foodmicrostructure/vol9/iss4/4