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Colorectal cancer is currently the third leading cause of cancer death in the world. The risk of developing colorectal cancer increases significantly in individuals who suffer from colitis, which is inflammation of the colon lining, seen in Irritable Bowel Disease. The term “leaky gut” is often used to describe increased intestinal permeability and is closely related to colitis. Leaky gut is associated with dysbiosis of the gut microbiota, which can occur through diet. This dysbiosis leads to pro-longed inflammation and affects genes that encode for proteins involved in the tight junctions between cells. Compromised intestinal integrity allows for translocation of material from the lumen of the colon, including bacteria, into the adjacent connective tissues and nearby blood supply. In this study we used the total Western diet, which emulates typical American dietary patterns at both macronutrient and micronutrient levels. Black raspberries were used as supplements to determine if the anti-inflammatory bioactives they contain would have a protective effect on the colon epithelium. The objective of this study is to determine how diet and supplementation of black raspberries affects expression of genes involved in intestinal permeability before, during, and after colitis. We hypothesize that expression of genes critical for maintaining the gut barrier and responding to bacteria infiltration will be differentially expressed in mice fed the total Western diet as compared to the AIN healthy diet, and their expression restored when supplemented with BRB.

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Logan, UT


colorectal cancer, colitis, leaky gut, microbiota



Effect of Basal Diet and Black Raspberry Supplementation on Gene Biomarkers of

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