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In tissue engineering, scaffolds are vital materials for cell growth. Scaffolds are used to provide the structural support for cell attachment and eventually tissue development. Although scaffold properties need to change to suit the nature and properties of the cells, most scaffolds need to simulate the extracellular matrix of the cells. Collagen is a major component of the extracellular matrix (ECM) in most tissues. Therefore, collagen is a favorable scaffold material for most tissues, including muscle tissue. However, collagen has poor mechanical properties; it is sometimes combined with synthetic polymers such as poly-Lactic Acid (PLA) for structural support. Furthermore, poly(dimethyl siloxane) (PDMS) has been previously used in scaffolds and has been noted for its flexibility and strong mechanical properties. The goal of this project is to explore several ways to apply collagen to a PDMS scaffold to increase the proliferation and viability of the cells as well as give the scaffold a stronger mechanical property than pure collagen scaffold would. Our hypothesis was that the PDMS crosslinked to collagen would provide the highest C2C12 cell proliferation compared to a pure PDMS scaffold or a pure collagen scaffold. We used two methods to combine the PDMS and collagen: cross-linking and cross-hatching. In a series of 12-well plates, we cultured C2C12 cells on both plasma-treated and untreated PDMS/collagen samples. Plasma-treated PDMS cross-hatched with collagen showed the highest cell proliferation among the samples and most closely matched the proliferation of an empty control well.

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Logan, UT


collagen, extracellular matrix, muscle tissue, PDMS scaffold



Collagen and PDMS Scaffolds for C2C12 Muscle Tissue Cell Line

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