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Scaffolds used in in vitro cellular growth are a common method of more accurately representing the structure and extracellular matrix of the tissue being grown. Tissue engineers are constantly testing novel biomaterials as scaffolds to determine their representativity of native tissue. One of these such biomaterials is hagfish protein, which when bound together or cross-linked has properties similar to many tissues found in the body. Specifically, there are some properties that could make it a viable option for human blood vessels. Being a simpler organ than most, comparatively, it is also a prime selection for in vitro studies attempting to create a transplantable tissue. Therefore, this experiment tests the viability of hagfish protein as a scaffod for blood vessels. Two cell lines were used in this study: C2C12s, a murine muscle cell derivative, and Human Umbilical Vein Endothelial Cells (HUVECs). They were seeded on a 12-well plate that had four treatments: two variations of Hagfish membrane, collagen as a positive control, and the untreated well plate as a negative control.

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Logan, UT


hagfish protein, human blood vessels, in vitro cellular growth, murine muscle cells



Tissue Engineered Representation of Human Blood Vessel Using Hagfish Protein

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