Identification of DNA Markers in Triticum aestivum-Aegilops caudata Additions Lines by Randomly Amplified Polymorphic DNA (RAPD) Technology

Ling Wei

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The objective of this study was to identify DNA markers for each of six added C-genome chromosomes in Triticum aestivum L. cv. 'Alceso'-Aegilops caudata L. addition lines using the randomly amplified polymorphic DNA (RAPD technique. DNA from Ae. caudata, T. aestivum, amphiploid of T.aestivum X Ae. caudata, and six disomic addition lines of what having a pair of Ae. caudata chromosomes was used as the template for the amplification of RAPD markers with a total of 58 random 10-mer oligonucleotide primers. Two primers, OPC-08 and OPJ-16, produced one intense band each from the amphiploid of T.aestivum X Ae.caudata and Ae. caudata, which was absent in all six addition lines. Each of these two primers produced a chromosome marker that could be tentatively located to the chromosome CA of Ae. caudata. OPJ-02, OPD-12, OPD-02, OPJ-12, OPD-20, and OPJ-14 produced a marker each for C8,CC,CD,CE,CF, and CG, respectively. OPJ-09 produced C-genome chromosome-specific RAPD markers and OPD-18 and OPD-19 produced wheat-specific RAPD markers. Also, OPC-05 and OPJ-19 produced RAPDs from both wheat and Ae. caudata genomes.